Academic Catalog

GENE405 ANIMAL CELL CULTURE TECHNIQUES

Course Code: 2190405
METU Credit (Theoretical-Laboratory hours/week): 4(2-4)
ECTS Credit: 6.0
Department: Molecular Biology And Genetics
Language of Instruction: English
Level of Study: Undergraduate
Course Coordinator: Prof.Dr. MESUDE CAN
Offered Semester: Fall or Spring Semesters.
Prerequisite: Set 1: 2380101
Set 2: 2190103 | 2190104
One of the sets above should be completed before taking GENE405 ANIMAL CELL CULTURE TECHNIQUES .

Course Content

This course aims to guide the students systematically through the fundamental principles of animal cell culture. It will also provide useful information for setting up a cell culture laboratory. Students will learn sterilization techniques, culture media preparation, basic animal cell culture techniques, microscopy of living cells, preparation of primary cell cultures, isolation of new cell lines, growing specialized cells in defined conditions, cell fusion, cloning, maintaining and controlling the quality of cell stocks, prevention and control of contamination and good laboratory practice.